Yesterday, Dr. Julie Horvath introduced you to our Armpit Microbe Pilot Study, known as #PitStart to those of you following along in real time on Twitter. Before we start asking thousands of you to send in smelly Q-tip’s that you’ve twirled around in your armpit, we need to work out a few methods. Namely, we’re concerned that some deodorants and antiperspirants inhibit bacterial growth – this was Julie’s personal experience when she tried to plate out her armpit microbes a few months ago.
So Dr. Julie H. and Dr. Jul Urban, the Assistant Director of the Genomics & Microbiology Research Lab at the Nature Research Center (NRC) at the NC Museum of Natural Sciences, and their trusty lab interns recruited 18 people, 9 men and 9 women, to go without deodorant or antiperspirant for a week. Full disclosure: I’m a participant.
On Sunday, participants went about their normal personal hygiene routines, applying deodorant or antiperspirant as per usual. Yesterday, we started the ‘Au Naturel’ treatment and will continue to go without deodorant/antiperspirant for the rest of the week. Every day between 11a and 1p, we have to sample each armpit with a sterile swab. Protocol calls for you to twirl the dual-tipped swabs in the deepest, wettest part of the armpit for 45 seconds. That’s longer than you think – Ok, at the risk of sharing too much information, here’s another disclosure: I take my phone in the bathroom with me and use the stopwatch app to time myself while swabbing. 45 seconds is an unbelievably long time.
Each day participants then deliver the day’s swabs directly to the Genomics & Microbio Lab at the NRC. There, members of Team Armpit dip and vigorously twirl a tip from each swab in a vial of liquid buffer to shake off the microbes. They then use a pipette to suck up a small volume of that microbe-laden liquid and spread it across a plate filled with nutrient-rich agar. The plates are plopped into a 37 degrees Celsius (or 99 degrees Fahrenheit, aka body temperature) incubator for about 24 hours, and we wait for the bacterial colonies to grow (or not).
Today, when I delivered my samples to the NRC, I had a chance to take a peek at the armpit microbes I collected on Day 0 of the experiment (the last day I wore antiperspirant).
Lucky for you, I brought my audio recorder with me. Here I am talking with Dr. Jul Urban:
Did you catch that? For Day 0, the plate from my right armpit barely had any bacterial colonies growing. Yet for the same day, the plate from my left armpit was FULL of bacteria! I took some pictures so you can see for yourself.